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KMID : 0903619910320030419
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Journal of the Korean Society for Horticultural Science
1991 Volume.32 No. 3 p.419 ~ p.423
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Detection of Odontoglossum Ringspot Virus by Enzyme - linked Immunosorbent Assay in Cymbidium Orchids
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Abstract
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Enzyme-linked immunosorbent assay (ELISA) with double antibody sandwich method succesefully detected odontoglossum ringspot virus (ORSV) in Cymbidium orchids. The symptoms caused by ORSV on the Cymbidium orchids were mosaic and necrotic spot. The effective concentration of γ-globulin and enzyme-labelled γ-globulin for detecting ORSV was 1㎍/㎖, and 2㎍/㎖, respectively. The sensitivity of the test, as determined by visual assessment and photometric measurement of the color reactions in serial dilution, allowed reliable detection of the virus in leaves of Cymbidium orchids. Crude leaf extracts taken from the ORSV infected Cymbidium were used as an antigen, the ELISA test detected as low as 1 : 5,120(w/v) dilution of sample. The incidence of virus disease was 69% in average. The occurences of virus disease in Cymbidium spp. and C. ensifolium mere mach higher than those of C. goeringii and C. kanran. Primary infection of ORSV could be readily detected in this method. Large differences in virus concentration were observed from individual Cymbidium orchids. ELISA was suitable and valuable method in screening for the detection of ORSV from the Cymbidium orchids.
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